Thymoquinone effects on autophagy, apoptosis, and oxidative stress in cisplatin-induced testicular damage in mice.
J Assist Reprod Genet
; 41(7): 1881-1891, 2024 Jul.
Article
in En
| MEDLINE
| ID: mdl-38568464
ABSTRACT
PURPOSE:
In this study, the effect of thymoquinone (TQ) on CP-induced spermatogenesis defects in mice has been investigated.METHODS:
Sperm parameters, serum testosterone concentration, histology, Bax/Bcl-2 ratio, and expression of autophagy-related biomarkers have been assessed. Total antioxidant capacity (TAC), total oxidant status (TOS), and oxidative stress index (OSI) in testicular tissue were examined for the evaluation of oxidative stress levels.RESULTS:
CP has induced histological changes and significantly increased the Bax/Bcl-2 ratio, decreased testosterone concentration, testicular weight, and sperm quality. CP induced oxidative stress by elevating OSI in the testicular tissue (p < 0.05). Expression of the autophagy-inducer genes (ATG7, ATG5, and Beclin-1) and ratio of LC3B/LC3A proteins were significantly decreased, while mTOR expression was increased in the CP group. TQ pretreatment dose-dependently decreased the Bax/Bcl-2 ratio and mTOR gene expression while increasing the expression of ATG5 and ATG7 genes, LC3B/LC3A ratio, and Beclin-1 proteins. TQ could also dose-dependently reverse the histology, testosterone level, and sperm quality of the CP-intoxicated mice.CONCLUSIONS:
These findings show that TQ pretreatment can enhance sperm production by inducing autophagy and reducing apoptosis and oxidative stress in the CP-intoxicated mouse testicles.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Spermatozoa
/
Autophagy
/
Testis
/
Benzoquinones
/
Cisplatin
/
Apoptosis
/
Oxidative Stress
Limits:
Animals
Language:
En
Journal:
J Assist Reprod Genet
Journal subject:
GENETICA
/
MEDICINA REPRODUTIVA
Year:
2024
Document type:
Article
Affiliation country:
Iran
Country of publication:
Netherlands