Protein purification, crystallization, and structure determination of transcription factor YhaJ in complex with DNT metabolites.
STAR Protoc
; 5(2): 102999, 2024 Jun 21.
Article
in En
| MEDLINE
| ID: mdl-38573865
ABSTRACT
The microbial transcription factor YhaJ responds to 2,4-dinitrotoluene (DNT) derivatives. Here, we describe steps for overexpression and purification of the protein, characterization for the binding of a DNT derivative methylhydroquinone, and crystallization by using a random seeding technique. We then detail procedures for structure determination by employing the crystal-twin resolving processes. This protocol can also be performed using other DNT derivatives. For complete details on the use and execution of this protocol, please refer to Kim et al.1.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Crystallization
Language:
En
Journal:
STAR Protoc
Year:
2024
Document type:
Article
Country of publication:
United States