Chemical bonding of cross-linked glutaraldehyde/chitosan on the surface of a titanium wire to prepare a robust biocompatible SPME fiber for analysis of phytohormones in plants.
Food Chem
; 449: 139168, 2024 Aug 15.
Article
in En
| MEDLINE
| ID: mdl-38574521
ABSTRACT
A robust biocompatible solid-phase microextraction (SPME) fiber, so-called Ti/APTS/GA/CS, was prepared by chemical bonding of cross-linked glutaraldehyde-chitosan to the surface of a titanium wire using APTS. The fiber was applied for sampling of phytohormones in plant tissues, followed by HPLC-UV analysis. The structure and morphology of the fiber coating was investigated by FT-IR, SEM, EDX, XRD, and TGA techniques. A Box-Behnken design was implemented to optimize the experimental variables. The calibration graphs were linear over a wide linear range (0.5-200 µg L-1) with LODs over the range of 0.01-0.06 µg L-1. The intra-day and inter-day precisions were found to be 1.3-6.3% and 4.3-7.3%, respectively. The matrix effect values ranged from 86.5% to 111.7%, indicating that the complex sample matrices had an insignificant effect on the determination of phytohormones. The fiber was successfully employed for the direct-immersion SPME (DI-SPME-HPLC) analysis of the phytohormones in cucumber, tomato, date palm, and calendula samples.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plant Growth Regulators
/
Titanium
/
Glutaral
/
Chitosan
/
Solid Phase Microextraction
Language:
En
Journal:
Food Chem
Year:
2024
Document type:
Article
Affiliation country:
Iran
Country of publication:
United kingdom