CDK activity at the centrosome regulates the cell cycle.
Cell Rep
; 43(4): 114066, 2024 Apr 23.
Article
in En
| MEDLINE
| ID: mdl-38578823
ABSTRACT
In human cells and yeast, an intact "hydrophobic patch" substrate docking site is needed for mitotic cyclin centrosomal localization. A hydrophobic patch mutant (HPM) of the fission yeast mitotic cyclin Cdc13 cannot enter mitosis, but whether this is due to defective centrosomal localization or defective cyclin-substrate docking more widely is unknown. Here, we show that artificially restoring Cdc13-HPM centrosomal localization promotes mitotic entry and increases CDK (cyclin-dependent kinase) substrate phosphorylation at the centrosome and in the cytoplasm. We also show that the S-phase B-cyclin hydrophobic patch is required for centrosomal localization but not for S phase. We propose that the hydrophobic patch is essential for mitosis due to its requirement for the local concentration of cyclin-CDK with CDK substrates and regulators at the centrosome. Our findings emphasize the central importance of the centrosome as a hub coordinating cell-cycle control and explain why the cyclin hydrophobic patch is essential for mitosis.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Schizosaccharomyces
/
Cell Cycle
/
Cyclin-Dependent Kinases
/
Centrosome
/
Cyclin B
/
Schizosaccharomyces pombe Proteins
/
Mitosis
Limits:
Humans
Language:
En
Journal:
Cell Rep
Year:
2024
Document type:
Article
Country of publication:
United States