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Fluorogenic cell surface glycan labelling with fluorescence molecular rotor dyes and nucleic acid stains.
Koçak, Alen; Homer, Amal K; Feida, Antonia; Telschow, Florian; Gorenflos López, Jacob L; Baydaroglu, Cihan; Gradzielski, Michael; Hackenberger, Christian P R; Alexiev, Ulrike; Seitz, Oliver.
Affiliation
  • Koçak A; Department of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Straße 2, 12489 Berlin, Germany. oliver.seitz@chemie.hu-berlin.de.
  • Homer AK; Department of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Straße 2, 12489 Berlin, Germany. oliver.seitz@chemie.hu-berlin.de.
  • Feida A; Department of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Straße 2, 12489 Berlin, Germany. oliver.seitz@chemie.hu-berlin.de.
  • Telschow F; Institut für Experimentalphysik, Freie Universität Berlin, Arnimallee 14, 14195 Berlin, Germany.
  • Gorenflos López JL; Leibniz-Forschungsinstitut für Molekulare Pharmakologie, Robert-Rössle-Straße 10, 13125 Berlin, Germany.
  • Baydaroglu C; Institute of Chemistry, Technische Universität Berlin, Straße des 17. Juni 124, 10623 Berlin, Germany.
  • Gradzielski M; Institute of Chemistry, Technische Universität Berlin, Straße des 17. Juni 124, 10623 Berlin, Germany.
  • Hackenberger CPR; Department of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Straße 2, 12489 Berlin, Germany. oliver.seitz@chemie.hu-berlin.de.
  • Alexiev U; Leibniz-Forschungsinstitut für Molekulare Pharmakologie, Robert-Rössle-Straße 10, 13125 Berlin, Germany.
  • Seitz O; Institut für Experimentalphysik, Freie Universität Berlin, Arnimallee 14, 14195 Berlin, Germany.
Chem Commun (Camb) ; 60(36): 4785-4788, 2024 Apr 30.
Article in En | MEDLINE | ID: mdl-38602157
ABSTRACT
We show that covalent labelling of sialic acids on live cell surfaces or mucin increases the fluorescence of the fluorescence molecular rotors (FMRs) CCVJ, Cy3 and thioazole orange, enabling wash-free imaging of cell surfaces. Dual labelling with an FMR and an environmentally insensitive dye allows detection of changes that occur, for example, when cross-linking is altered.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Fluorescent Dyes Limits: Humans Language: En Journal: Chem Commun (Camb) Journal subject: QUIMICA Year: 2024 Document type: Article Affiliation country: Germany Country of publication: United kingdom
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Fluorescent Dyes Limits: Humans Language: En Journal: Chem Commun (Camb) Journal subject: QUIMICA Year: 2024 Document type: Article Affiliation country: Germany Country of publication: United kingdom