Analytical Methods for Brassinosteroid Analysis: Recent Advances and Applications.
Plant Cell Physiol
; 2024 Apr 15.
Article
in En
| MEDLINE
| ID: mdl-38619131
ABSTRACT
Brassinosteroids (BRs) are plant steroidal hormones that play crucial roles in plant growth and development. Accurate quantification of BRs in plant tissues is essential for understanding their biological functions. This study presents a comprehensive overview of the latest methods used for the quantification of BRs in plants. We discuss the principles, advantages, and limitations of various analytical techniques, including immunoassays, gas chromatography-mass spectrometry (GC-MS), and liquid chromatography-tandem mass spectrometry (LC-MS/MS) that are used for the detection and quantification of BRs from complex plant matrices. We also explore the use of isotopically labeled internal standards to improve the accuracy and reliability of BR quantification.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Plant Cell Physiol
Journal subject:
BOTANICA
Year:
2024
Document type:
Article
Affiliation country:
Czech Republic