RUNX transcription factors are essential in maintaining epididymal epithelial differentiation.
Cell Mol Life Sci
; 81(1): 183, 2024 Apr 17.
Article
in En
| MEDLINE
| ID: mdl-38630262
ABSTRACT
Apart from the androgen receptor, transcription factors (TFs) that are required for the development and formation of the different segments of the epididymis have remained unknown. We identified TF families expressed in the developing epididymides, of which many showed segment specificity. From these TFs, down-regulation of runt related transcription factors (RUNXs) 1 and 2 expression coincides with epithelial regression in Dicer1 cKO mice. Concomitant deletion of both Runx1 and Runx2 in a mouse epididymal epithelial cell line affected cell morphology, adhesion and mobility in vitro. Furthermore, lack of functional RUNXs severely disturbed the formation of 3D epididymal organoid-like structures. Transcriptomic analysis of the epididymal cell organoid-like structures indicated that RUNX1 and RUNX2 are involved in the regulation of MAPK signaling, NOTCH pathway activity, and EMT-related gene expression. This suggests that RUNXs are master regulators of several essential signaling pathways, and necessary for the maintenance of proper differentiation of the epididymal epithelium.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Core Binding Factor Alpha 1 Subunit
/
Core Binding Factor Alpha 2 Subunit
Limits:
Animals
/
Humans
/
Male
Language:
En
Journal:
Cell Mol Life Sci
Journal subject:
BIOLOGIA MOLECULAR
Year:
2024
Document type:
Article
Affiliation country:
Finland