Effect of 2-aminoethoxydiphenyl borate on the functions of mouse skeletal muscle mitochondria.
Biochem Biophys Res Commun
; 712-713: 149944, 2024 Jun 18.
Article
in En
| MEDLINE
| ID: mdl-38636302
ABSTRACT
This work examined the effect of 2-aminoethoxydiphenyl borate (2-APB) on the functioning of isolated mouse skeletal muscle mitochondria and modeled its putative interaction with mitochondrial proteins. We have shown that 2-APB is able to dose-dependently suppress mitochondrial respiration in state 3 and 3UDNP driven by substrates of complex I and II. This effect of 2-APB was accompanied by a slight dose-dependent decrease in mitochondrial membrane potential and appears to be due to inhibition of complex I and complex III of the electron transport chain (ETC) with IC50 values of 200 and 120 µM, respectively. The results of molecular docking identified putative 2-APB interaction sites in these ETC complexes. 2-APB was shown to dose-dependently inhibit both mitochondrial Ca2+ uptake and Ca2+ efflux, which seems to be caused by a decrease in the membrane potential of the organelles. We have found that 2-APB has no significant effect on mitochondrial calcium retention capacity. On the other hand, 2-APB exhibited antioxidant effect by reducing mitochondrial hydrogen peroxide production but without affecting superoxide generation. It is concluded that the effect of 2-APB on mitochondrial targets should be taken into account when interpreting the results of cell and in vivo experiments.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Boron Compounds
/
Calcium
/
Muscle, Skeletal
/
Mitochondria, Muscle
Limits:
Animals
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
2024
Document type:
Article