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Tumor necrosis factor receptor-associated factor 5 protects against intimal hyperplasia by regulation of macrophage polarization via directly targeting PPARγ.
Cheng, Wen-Lin; Chao, Sheng-Ping; Zhao, Fang; Cai, Huan-Huan; Zeng, Ziyue; Cao, Jian-Lei; Jin, Zhili; Deng, Ke-Qiong; Hu, Xiaorong; Wang, Hairong; Lu, Zhibing.
Affiliation
  • Cheng WL; Department of Cardiology, Zhongnan Hospital, Wuhan University, No.169 Donghu Road, Wuchang District, Wuhan, 430071, China.
  • Chao SP; Institute of Myocardial Injury and Repair, Wuhan University, Wuhan, 430071, China.
  • Zhao F; Department of Cardiology, Zhongnan Hospital, Wuhan University, No.169 Donghu Road, Wuchang District, Wuhan, 430071, China.
  • Cai HH; Institute of Myocardial Injury and Repair, Wuhan University, Wuhan, 430071, China.
  • Zeng Z; Department of Cardiology, Zhongnan Hospital, Wuhan University, No.169 Donghu Road, Wuchang District, Wuhan, 430071, China.
  • Cao JL; Institute of Myocardial Injury and Repair, Wuhan University, Wuhan, 430071, China.
  • Jin Z; Department of Cardiology, Zhongnan Hospital, Wuhan University, No.169 Donghu Road, Wuchang District, Wuhan, 430071, China.
  • Deng KQ; Institute of Myocardial Injury and Repair, Wuhan University, Wuhan, 430071, China.
  • Hu X; Department of Cardiology, Zhongnan Hospital, Wuhan University, No.169 Donghu Road, Wuchang District, Wuhan, 430071, China.
  • Wang H; Institute of Myocardial Injury and Repair, Wuhan University, Wuhan, 430071, China.
  • Lu Z; Department of Cardiology, Zhongnan Hospital, Wuhan University, No.169 Donghu Road, Wuchang District, Wuhan, 430071, China.
Inflamm Res ; 73(6): 929-943, 2024 Jun.
Article in En | MEDLINE | ID: mdl-38642079
ABSTRACT

OBJECTIVES:

Intimal hyperplasia is a serious clinical problem associated with the failure of therapeutic methods in multiple atherosclerosis-related coronary heart diseases, which are initiated and aggravated by the polarization of infiltrating macrophages. The present study aimed to determine the effect and underlying mechanism by which tumor necrosis factor receptor-associated factor 5 (TRAF5) regulates macrophage polarization during intimal hyperplasia.

METHODS:

TRAF5 expression was detected in mouse carotid arteries subjected to wire injury. Bone marrow-derived macrophages, mouse peritoneal macrophages and human myeloid leukemia mononuclear cells were also used to test the expression of TRAF5 in vitro. Bone marrow-derived macrophages upon to LPS or IL-4 stimulation were performed to examine the effect of TRAF5 on macrophage polarization. TRAF5-knockout mice were used to evaluate the effect of TRAF5 on intimal hyperplasia.

RESULTS:

TRAF5 expression gradually decreased during neointima formation in carotid arteries in a time-dependent manner. In addition, the results showed that TRAF5 expression was reduced in classically polarized macrophages (M1) subjected to LPS stimulation but was increased in alternatively polarized macrophages (M2) in response to IL-4 administration, and these changes were demonstrated in three different types of macrophages. An in vitro loss-of-function study with TRAF5 knockdown plasmids or TRAF5-knockout mice revealed high expression of markers associated with M1 macrophages and reduced expression of genes related to M2 macrophages. Subsequently, we incubated vascular smooth muscle cells with conditioned medium of polarized macrophages in which TRAF5 expression had been downregulated or ablated, which promoted the proliferation, migration and dedifferentiation of VSMCs. Mechanistically, TRAF5 knockdown inhibited the activation of anti-inflammatory M2 macrophages by directly inhibiting PPARγ expression. More importantly, TRAF5-deficient mice showed significantly aggressive intimal hyperplasia.

CONCLUSIONS:

Collectively, this evidence reveals an important role of TRAF5 in the development of intimal hyperplasia through the regulation of macrophage polarization, which provides a promising target for arterial restenosis-related disease management.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Mice, Knockout / TNF Receptor-Associated Factor 5 / PPAR gamma / Hyperplasia / Macrophages / Mice, Inbred C57BL Limits: Animals / Humans / Male Language: En Journal: Inflamm Res Journal subject: ALERGIA E IMUNOLOGIA / PATOLOGIA Year: 2024 Document type: Article Affiliation country: China Country of publication: Switzerland

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Mice, Knockout / TNF Receptor-Associated Factor 5 / PPAR gamma / Hyperplasia / Macrophages / Mice, Inbred C57BL Limits: Animals / Humans / Male Language: En Journal: Inflamm Res Journal subject: ALERGIA E IMUNOLOGIA / PATOLOGIA Year: 2024 Document type: Article Affiliation country: China Country of publication: Switzerland