A murine model of DC-SIGN humanization exhibits increased susceptibility against SARS-CoV-2.
Microbes Infect
; 26(5-6): 105344, 2024.
Article
in En
| MEDLINE
| ID: mdl-38670218
ABSTRACT
To generate a new murine model for virus, DC-SIGN gene in murine was humanized. In this study, we successfully generated a humanized C57BL/6N mouse model expressing human DC-SIGN (hDC-SIGN) using CRISPR/Cas9 technology, and evaluated its characters and susceptibility to virus. The humanized mice could survival as usual, and with normal physiological index just like the wild-type mice. Whereas, we found significant differences in the intestinal flora and metabolic profiles between wild-type mice and humanized mice. Following intranasal infection with SARS-CoV-2, hDC-SIGN mice exhibited significantly increased viral loads in the lungs and nasal turbinates, along with more severe lung damage. This phenomenon may be associated with differential lipid metabolism and Fcγ receptor-mediated phagocytosis in two mouse models. This study provides a useful tool for investigating the mechanisms of coronavirus infection and potential drug therapies against novel coronavirus.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cell Adhesion Molecules
/
Receptors, Cell Surface
/
Lectins, C-Type
/
Disease Models, Animal
/
SARS-CoV-2
/
COVID-19
/
Mice, Inbred C57BL
Limits:
Animals
/
Humans
Language:
En
Journal:
Microbes Infect
Journal subject:
ALERGIA E IMUNOLOGIA
/
MICROBIOLOGIA
Year:
2024
Document type:
Article
Affiliation country:
China