The effects of NLRP3 inflammasome inhibition or knockout in experimental apical periodontitis induced in mice.
Clin Oral Investig
; 28(5): 285, 2024 Apr 30.
Article
in En
| MEDLINE
| ID: mdl-38684528
ABSTRACT
OBJECTIVE:
To evaluate the effects of NLRP3 inflammasome inhibition or knockout in experimental apical periodontitis (AP) induced in mice.METHODS:
The experimental AP was induced by pulpal exposure. To evaluate NLRP3-specific inhibitor medication (MCC950), WT mice received intraperitoneal injections, while the control received PBS (n = 10). In addition, to evaluate NLRP3 knockout, 35 wild-type (WT) and 35 NLRP3-/- mice were divided into a control group (without pulpal exposure, n = 5) and three experimental groups after 2, 14 and 42 days after pulpal exposure (n = 10). Microscopic and molecular analyzes were carried out using a significance level of 5%.RESULTS:
Exposure to MCC950 did not affect the periapical lesion size after 14 days (P = 0.584). However, exposed mice had a lower expression of IL-1ß, IL-18 and caspase-1 (P = 0.010, 0.016 and 0.002, respectively). Moreover, NLRP3-/- mice showed a smaller periapical lesion after 14 and 42 days (P = 0.023 and 0.031, respectively), as well as a lower expression of IL-1ß after 42 days (P < 0.001), of IL-18 and caspase-1 after 14 (P < 0.001 and 0.035, respectively) and 42 days (P = 0.002 and 0.002, respectively). NLRP3-/- mice also showed a lower mRNA for Il-1ß, Il-18 and Casp1 after 2 (P = 0.002, 0.036 and 0.001, respectively) and 14 days (P = 0.002, 0.002 and 0.001, respectively).CONCLUSIONS:
NLRP3 inflammasome inhibition or knockout can attenuate the inflammatory events that result in the periapical lesion (AP) formation after pulpal exposure in mice. CLINICAL RELEVANCE The NLRP3 inflammasome may be a therapeutic target for AP, and new approaches may verify the impact of its inhibition (through intracanal medications or filling materials) on the bone repair process and treatment success.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Periapical Periodontitis
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Mice, Knockout
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Disease Models, Animal
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Inflammasomes
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NLR Family, Pyrin Domain-Containing 3 Protein
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Indenes
Limits:
Animals
Language:
En
Journal:
Clin Oral Investig
Journal subject:
ODONTOLOGIA
Year:
2024
Document type:
Article
Affiliation country:
Brazil