Modulating intestinal health: Impact of chitooligosaccharide molecular weight on suppressing RAGE expression and inflammatory response in methylglyoxal-induced advanced glycation end-products.
Int J Biol Macromol
; 269(Pt 2): 131927, 2024 Jun.
Article
in En
| MEDLINE
| ID: mdl-38685538
ABSTRACT
The accumulation of methylglyoxal (MGO) produced in high-temperature processed foods and excessive production in the body contributes to intestinal barrier dysfunction. In this study, we investigated the effects of chitooligosaccharides (COSs) of different molecular weights (<1â¯kDa, 1-3â¯kDa, 3-5â¯kDa, 5-10â¯kDa, and >10â¯kDa) on MGO-induced intestinal barrier dysfunction. We investigated the effect of COSs on inhibiting intracellular MGO accumulation/MGO-derived AGEs production and regulating the receptor for AGE (RAGE)-mediated downstream protein expression, including proteins related to apoptosis and inflammation, intestinal barrier integrity, and paracellular permeability. Pretreatment with COSs ameliorated MGO-induced increased RAGE protein expression, activation of apoptotic cascade/inflammatory response, loss of intestinal epithelial barrier integrity, and increased paracellular permeability, ameliorating intestinal dysfunction through MGO scavenging. 1-3â¯kDa COSs most effectively ameliorated MGO-induced intestinal dysfunction. Our results suggest the potential of COSs in improving intestinal health by ameliorating intestinal barrier dysfunction by acting as an MGO scavenger and highlighting the need for the optimization of the molecular weight of COSs to optimize its protective effects.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oligosaccharides
/
Pyruvaldehyde
/
Glycation End Products, Advanced
/
Chitosan
/
Receptor for Advanced Glycation End Products
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Intestinal Mucosa
/
Molecular Weight
Limits:
Animals
/
Humans
Language:
En
Journal:
Int J Biol Macromol
Year:
2024
Document type:
Article
Country of publication:
Netherlands