Streptomyces albipurpureus sp. nov., a novel actinobacterium with antimicrobial activity isolated from the rhizospheric soil of Fritillaria cirrhosa D. Don.
Int J Syst Evol Microbiol
; 74(5)2024 May.
Article
in En
| MEDLINE
| ID: mdl-38695864
ABSTRACT
A novel actinobacterium, designated strain CWNU-1T, was isolated from the rhizospheric soil of Fritillaria cirrhosa D. Don and examined using a polyphasic taxonomic approach. The organism developed pale blue aerial mycelia that was simply branched and terminated in open or closed coils of three or more volutions on International Streptomyces Project 3 agar. Spores were ellipsoidal to cylindrical with wrinkled surfaces. The strain showed high 16S rRNA gene sequence similarity to Streptomyces kurssanovii NBRC 13192T (98.8â%), Streptomyces xantholiticus NBRC 13354T (98.7â%) and Streptomyces peucetius JCM 9920T (98.6â%). The phylogenetic result based on 16S rRNA gene and genome sequences clearly demonstrated that strain CWNU-1T formed an independent phylogenetic lineage. On the basis of orthologous average nucleotide identity, CWNU-1T was most closely related to Streptomyces inusitatus NBRC 13601T with 79.3â% identity. The results of the digital DNA-DNA hybridization analysis also indicated low levels of relatedness with other species, as the highest value was observed with S. inusitatus NBRC 13601T (25.3â%). With reference to phenotypic characteristics, phylogenetic data, orthologous average nucleotide identity and digital DNA-DNA hybridization results, strain CWNU-1T was readily distinguished from its most closely related strains and classified as representing a novel species, for which the name Streptomyces albipurpureus sp. nov. is proposed. The type strain is CWNU-1T (=CGMCC 4.7758T=MCCC 1K07402T=JCM 35391T).
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phylogeny
/
Soil Microbiology
/
Streptomyces
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Base Composition
/
DNA, Bacterial
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RNA, Ribosomal, 16S
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Bacterial Typing Techniques
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Sequence Analysis, DNA
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Fritillaria
/
Fatty Acids
Language:
En
Journal:
Int J Syst Evol Microbiol
Journal subject:
MICROBIOLOGIA
Year:
2024
Document type:
Article
Country of publication:
United kingdom