Discovery of active mouse, plant and fungal cytochrome P450s in endogenous proteomes and upon expression in planta.
Sci Rep
; 14(1): 10091, 2024 05 02.
Article
in En
| MEDLINE
| ID: mdl-38698065
ABSTRACT
Eukaryotes produce a large number of cytochrome P450s that mediate the synthesis and degradation of diverse endogenous and exogenous metabolites. Yet, most of these P450s are uncharacterized and global tools to study these challenging, membrane-resident enzymes remain to be exploited. Here, we applied activity profiling of plant, mouse and fungal P450s with chemical probes that become reactive when oxidized by P450 enzymes. Identification by mass spectrometry revealed labeling of a wide range of active P450s, including six plant P450s, 40 mouse P450s and 13 P450s of the fungal wheat pathogen Zymoseptoria tritici. We next used transient expression of GFP-tagged P450s by agroinfiltration to show ER-targeting and NADPH-dependent, activity-based labeling of plant, mouse and fungal P450s. Both global profiling and transient expression can be used to detect a broad range of active P450s to study e.g. their regulation and discover selective inhibitors.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Fungal Proteins
/
Proteome
/
Cytochrome P-450 Enzyme System
Limits:
Animals
Language:
En
Journal:
Sci Rep
Year:
2024
Document type:
Article
Affiliation country:
United kingdom