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Exploring the extrachromosomal plasmid rDNA of Naegleria fowleri AY27 genotype II: A human brain-eating amoeba via high-throughput sequencing.
Aurongzeb, Muhammad; Talha Malik, Hafiz Muhammad; Jahanzaib, Muhammad; Hassan, Syed Shah; Rashid, Yasmeen; Aziz, Tariq; Alharbi, Metab.
Affiliation
  • Aurongzeb M; Department of Biotechnology, Faculty of Engineering Sciences & Technology, Hamdard University, Karachi, 74600, Pakistan.
  • Talha Malik HM; Alpha Genomics Private limited, Islamabad, Pakistan.
  • Jahanzaib M; JRC Genome Research, PCMD, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan.
  • Hassan SS; JRC Genome Research, PCMD, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan.
  • Rashid Y; Department of Biochemistry, University of Karachi, Karachi, 75270, Pakistan. yasmeen.rashid@uok.edu.pk.
  • Aziz T; Laboratory of Animal Health Food Hygiene and Quality, Department of Agriculture, University of Ioannina, Arta, 47132, Greece.
  • Alharbi M; Department of Pharmacology and Toxicology College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
BMC Med Genomics ; 17(1): 125, 2024 May 07.
Article in En | MEDLINE | ID: mdl-38715056
ABSTRACT
Naegleria fowleri, also known as brain-earing amoeba, causes severe and rapidly fatal CNS infection in humans called primary amebic meningoencephalitis (PAM). The DNA from the N. fowleri clinical isolate was sequenced for circular extrachromosomal ribosomal DNA (CERE - rDNA). The CERE contains 18 S, 5.8 S, and 28 S ribosomal subunits separated by internal transcribed spacers, 5 open reading frames (ORFs), and mostly repeat elements comprising 7268 bp out of 15,786 bp (46%). A wide variety of variations and recombination events were observed. Finally, the ORFs that comprised only 4 hypothetical proteins were modeled and screened against Zinc drug-like compounds. Two compounds [ZINC77564275 (ethyl 2-(((4-isopropyl-4 H-1,2,4-triazol-3-yl) methyl) (methyl)amino) oxazole-4-carboxylate) and ZINC15022129 (5-(2-methoxyphenoxy)-[2,2'-bipyrimidine]-4,6(1 H,5 H)-dione)] were finalized as potential druggable compounds based on ADME toxicity analysis. We propose that the compounds showing the least toxicity would be potential drug candidates after laboratory experimental validation is performed.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA, Ribosomal / Naegleria fowleri / High-Throughput Nucleotide Sequencing Limits: Humans Language: En Journal: BMC Med Genomics Journal subject: GENETICA MEDICA Year: 2024 Document type: Article Affiliation country: Pakistan Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA, Ribosomal / Naegleria fowleri / High-Throughput Nucleotide Sequencing Limits: Humans Language: En Journal: BMC Med Genomics Journal subject: GENETICA MEDICA Year: 2024 Document type: Article Affiliation country: Pakistan Country of publication: United kingdom