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On-demand expansion fluorescence and photoacoustic microscopy (ExFLPAM).
Mu, Xuan; Ma, Chenshuo; Mei, Xuan; Liao, Junlong; Bojar, Rebecca; Kuang, Sizhe; Rong, Qiangzhou; Yao, Junjie; Zhang, Yu Shrike.
Affiliation
  • Mu X; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 65 Landsdowne Street, Cambridge, MA 02139, USA.
  • Ma C; Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
  • Mei X; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 65 Landsdowne Street, Cambridge, MA 02139, USA.
  • Liao J; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 65 Landsdowne Street, Cambridge, MA 02139, USA.
  • Bojar R; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 65 Landsdowne Street, Cambridge, MA 02139, USA.
  • Kuang S; Barnard College, Columbia University, New York, NY 10027, USA.
  • Rong Q; Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
  • Yao J; Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
  • Zhang YS; Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
Photoacoustics ; 38: 100610, 2024 Aug.
Article in En | MEDLINE | ID: mdl-38726025
ABSTRACT
Expansion microscopy (ExM) is a promising technology that enables nanoscale imaging on conventional optical microscopes by physically magnifying the specimens. Here, we report the development of a strategy that enables i) on-demand labeling of subcellular organelles in live cells for ExM through transfection of fluorescent proteins that are well-retained during the expansion procedure; and ii) non-fluorescent chromogenic color-development towards efficient bright-field and photoacoustic imaging in both planar and volumetric formats, which is applicable to both cultured cells and biological tissues. Compared to the conventional ExM methods, our strategy provides an expanded toolkit, which we term as expansion fluorescence and photoacoustic microscopy (ExFLPAM), by allowing on-demand fluorescent protein labeling of cultured cells, as well as non-fluorescent absorption contrast-imaging of biological samples.
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Photoacoustics Year: 2024 Document type: Article Country of publication: Germany

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Photoacoustics Year: 2024 Document type: Article Country of publication: Germany