Microwave Flow Cytometric Detection and Differentiation of Escherichia coli.
Sensors (Basel)
; 24(9)2024 Apr 30.
Article
in En
| MEDLINE
| ID: mdl-38732977
ABSTRACT
Label-free measurement and analysis of single bacterial cells are essential for food safety monitoring and microbial disease diagnosis. We report a microwave flow cytometric sensor with a microstrip sensing device with reduced channel height for bacterial cell measurement. Escherichia coli B and Escherichia coli K-12 were measured with the sensor at frequencies between 500 MHz and 8 GHz. The results show microwave properties of E. coli cells are frequency-dependent. A LightGBM model was developed to classify cell types at a high accuracy of 0.96 at 1 GHz. Thus, the sensor provides a promising label-free method to rapidly detect and differentiate bacterial cells. Nevertheless, the method needs to be further developed by comprehensively measuring different types of cells and demonstrating accurate cell classification with improved machine-learning techniques.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Escherichia coli
/
Flow Cytometry
/
Microwaves
Language:
En
Journal:
Sensors (Basel)
Year:
2024
Document type:
Article
Affiliation country:
United States