CD22 blockade aggravates EAE and its role in microglia polarization.
CNS Neurosci Ther
; 30(5): e14736, 2024 05.
Article
in En
| MEDLINE
| ID: mdl-38739106
ABSTRACT
AIMS:
Multiple sclerosis (MS) is a neuroinflammatory demyelinating disease. Microglia are reportedly involved in the pathogenesis of MS. However, the key molecules that control the inflammatory activity of microglia in MS have not been identified.METHODS:
Experimental autoimmune encephalomyelitis (EAE) mice were randomized into CD22 blockade and control groups. The expression levels of microglial CD22 were measured by flow cytometry, qRT-PCR, and immunofluorescence. The effects of CD22 blockade were examined via in vitro and in vivo studies.RESULTS:
We detected increased expression of microglial CD22 in EAE mice. In addition, an in vitro study revealed that lipopolysaccharide upregulated the expression of CD22 in microglia and that CD22 blockade modulated microglial polarization. Moreover, an in vivo study demonstrated that CD22 blockade aggravated EAE in mice and promoted microglial M1 polarization.CONCLUSION:
Collectively, our study indicates that CD22 may be protective against EAE and may play a critical role in the maintenance of immune homeostasis in EAE mice.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Microglia
/
Encephalomyelitis, Autoimmune, Experimental
/
Sialic Acid Binding Ig-like Lectin 2
Limits:
Animals
Language:
En
Journal:
CNS Neurosci Ther
/
CNS neurosc. ther. (Print)
/
CNS neuroscience & therapeutics (Print)
Journal subject:
NEUROLOGIA
/
TERAPEUTICA
Year:
2024
Document type:
Article
Affiliation country:
China
Country of publication:
United kingdom