Molecular insights into the prototypical single-stranded DNA-binding protein from E. coli.
Crit Rev Biochem Mol Biol
; 59(1-2): 99-127, 2024.
Article
in En
| MEDLINE
| ID: mdl-38770626
ABSTRACT
The SSB protein of Escherichia coli functions to bind single-stranded DNA wherever it occurs during DNA metabolism. Depending upon conditions, SSB occurs in several different binding modes. In the course of its function, SSB diffuses on ssDNA and transfers rapidly between different segments of ssDNA. SSB interacts with many other proteins involved in DNA metabolism, with 22 such SSB-interacting proteins, or SIPs, defined to date. These interactions chiefly involve the disordered and conserved C-terminal residues of SSB. When not bound to ssDNA, SSB can aggregate to form a phase-separated biomolecular condensate. Current understanding of the properties of SSB and the functional significance of its many intermolecular interactions are summarized in this review.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA, Single-Stranded
/
Escherichia coli Proteins
/
DNA-Binding Proteins
/
Escherichia coli
Language:
En
Journal:
Crit Rev Biochem Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Year:
2024
Document type:
Article
Affiliation country:
United States