Facile green spectrophotometric approaches for the determination of three natural edible antioxidant polyphenols in different matrices.
Spectrochim Acta A Mol Biomol Spectrosc
; 318: 124428, 2024 Oct 05.
Article
in En
| MEDLINE
| ID: mdl-38781825
ABSTRACT
The combination of Curcumin (CRN), resveratrol (RSV), and quercetin (QRN) has significant antioxidant effects and is found to be more effective than a single polyphenol. Spectrophotometric methods are considered one of the most common analytical techniques for the determination of the drugs due to their sensitivity, rapidness, low cost, and reproducibility. Therefore, the presence of new, and simple methods for the determination of such compounds will be highly valuable, specially in the presence of spectral overlap. In this research, five different facile spectrophotometric methods were investigated for the simultaneous determination of that ternary mixture for the first time, including zero order (I), first derivative (II), ratio difference double divisor (III), first derivative ratio spectra (IV), and mean centering (V) methods. The designed approaches were linear over the concentration ranges of (1.0-10.0), (0.5-8.0), and (1.0-14.0) µg/mL, respectively for curcumin, resveratrol, and quercetin. The different methods were then validated as stated by the International Council of Harmonization. The accuracy and precision have been evaluated by statistical analysis including student t-test, variance ratio F-test, and ANOVA. Moreover, the greenness and whiteness of the proposed methods were assessed to ensure the adherence to the greenness characters.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Quercetin
/
Spectrophotometry
/
Curcumin
/
Polyphenols
/
Resveratrol
/
Antioxidants
Language:
En
Journal:
Spectrochim Acta A Mol Biomol Spectrosc
Journal subject:
BIOLOGIA MOLECULAR
Year:
2024
Document type:
Article
Affiliation country:
Egypt
Country of publication:
United kingdom