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Multiplex Assays in Allergy Diagnosis: Allergy Explorer 2 versus ImmunoCAP ISAC E112i.
Nösslinger, Hannes; Mair, Ewald; Oostingh, Gertie J; Ahlgrimm-Siess, Verena; Ringauf, Anna; Lang, Roland.
Affiliation
  • Nösslinger H; Department of Nutrition and Dietetics, Hospital of Merano (SABES-ASDAA), Lehrkrankenhaus der Paracelsus Medizinischen Privatuniversität, 39012 Merano-Meran, Italy.
  • Mair E; Registered pediatrician (SABES-ASDAA), 39031 Brunico-Bruneck, Italy.
  • Oostingh GJ; Biomedical Sciences, Salzburg University of Applied Sciences, 5431 Puch/Salzburg, Austria.
  • Ahlgrimm-Siess V; Department of Dermatology and Allergology, University Hospital of the Paracelsus Medical University, 5020 Salzburg, Austria.
  • Ringauf A; MacroArray Diagnostics (MADx), 1230 Vienna, Austria.
  • Lang R; Department of Dermatology and Allergology, University Hospital of the Paracelsus Medical University, 5020 Salzburg, Austria.
Diagnostics (Basel) ; 14(10)2024 May 08.
Article in En | MEDLINE | ID: mdl-38786274
ABSTRACT
ImmunoCAP ISAC E112i (ISAC) and Allergy Explorer 2 (ALEX2) detect specific immunoglobulin E (IgE) reactivity. Both multiplex assays contain molecular allergens and ALEX2 additionally includes allergen extracts and inhibitors that block the binding of IgE to cross-reacting carbohydrate determinants (CCDs). This study aimed to compare the performance of ISAC and ALEX2 by determining the IgE reactivity against allergen extracts and/or allergen components and by using qualitative, semiquantitative, and quantitative analyses of all comparable allergen components in sera from 216 participants recruited in South Tyrol/Italy. For extract sensitization in ALEX2, the analysis revealed negative corresponding allergen components in 18.4% and at least one positive corresponding allergen component in 81.6% of all cases. For ISAC, the corresponding results were 23.5% and 76.5% of cases, respectively. The ALEX2 CCD inhibitor eliminated CCD-positive signals detected by ISAC in 88.5% of cases. Based on sensitization values of 0.3-14.9 ISU or kUA/L, there was good agreement between ALEX2 and ISAC, although ALEX2 showed higher values than ISAC. The addition of allergen-extract tests in ALEX2 resulted in the detection of more sensitizations than with corresponding allergen components alone. In the range of <15 ISU or kUA/L, ALEX2 may be more effective in detecting sensitizations.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Diagnostics (Basel) Year: 2024 Document type: Article Affiliation country: Italy Country of publication: Switzerland

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Diagnostics (Basel) Year: 2024 Document type: Article Affiliation country: Italy Country of publication: Switzerland