Inhibition of α-glucosidase activity by potential peptides derived from fermented spent coffee grounds.
Food Chem
; 454: 139791, 2024 Oct 01.
Article
in En
| MEDLINE
| ID: mdl-38795616
ABSTRACT
The control of α-glucosidase activity has been associated with managing diabetes. We previously identified three peptides with high bioactive indices derived from protein hydrolysates of fermented spent coffee grounds. In this study, the peptides YGF, GMCC, and RMYRY were synthesized and tested in vitro for their α-glucosidase inhibition activity, complemented by in silico analyses. Two of the three peptides significantly inhibited α-glucosidase activity, with the more efficient peptides being YGF and GMCC (0.42 mg/mL), resulting in decreased enzymatic activity of 95.31% and 89.79%, respectively. These peptides exhibited binding free energies with the α-glucosidase complex of -8.5 and - 6.6 kcal/mol, respectively, through hydrogen bonds and van der Waals interactions with amino acids from the active site. Pharmacokinetic analysis indicated that YGF and GMCC profiles were unrelated to toxicity. These results underscore the importance of focusing on food waste bioprocessing products to expand the range of alternatives that could aid in diabetes treatment.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptides
/
Waste Products
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Alpha-Glucosidases
/
Fermentation
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Glycoside Hydrolase Inhibitors
Limits:
Animals
/
Humans
Language:
En
Journal:
Food Chem
Year:
2024
Document type:
Article