Evaluation of different molecular systems for detection and quantification of SARS-CoV-2 RNA from wastewater samples.
J Virol Methods
; 328: 114956, 2024 Jul.
Article
in En
| MEDLINE
| ID: mdl-38796134
ABSTRACT
Wastewater-based epidemiology has proved to be a suitable approach for tracking the spread of epidemic agents including SARS-CoV-2 RNA. Different protocols have been developed for quantitative detection of SARS-CoV-2 RNA from wastewater samples, but little is known on their performance. In this study we compared three protocols based on Reverse Transcription Real Time-PCR (RT-PCR) and one based on Droplet Digital PCR (ddPCR) for SARS-CoV-2 RNA detection from 35 wastewater samples. Overall, SARS-CoV-2 RNA was detected by at least one method in 85.7â¯% of samples, while 51.4â¯%, 22.8â¯% and 8.6â¯% resulted positive with two, three or all four methods, respectively. Protocols based on commercial RT-PCR assays and on Droplet Digital PCR showed an overall higher sensitivity vs. an in-house assay. The use of more than one system, targeting different genes, could be helpful to increase detection sensitivity.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
RNA, Viral
/
Sensitivity and Specificity
/
Wastewater
/
SARS-CoV-2
/
COVID-19
Limits:
Humans
Language:
En
Journal:
J Virol Methods
Year:
2024
Document type:
Article
Country of publication:
Netherlands