Atg44/Mdi1/mitofissin facilitates Dnm1-mediated mitochondrial fission.
Autophagy
; : 1-9, 2024 Jun 04.
Article
in En
| MEDLINE
| ID: mdl-38818923
ABSTRACT
Mitochondria undergo fission and fusion, and their coordinated balance is crucial for maintaining mitochondrial homeostasis. In yeast, the dynamin-related protein Dnm1 is a mitochondrial fission factor acting from outside the mitochondria. We recently reported the mitochondrial intermembrane space protein Atg44/mitofissin/Mdi1/Mco8 as a novel fission factor, but the relationship between Atg44 and Dnm1 remains elusive. Here, we show that Atg44 is required to complete Dnm1-mediated mitochondrial fission under homeostatic conditions. Atg44-deficient cells often exhibit enlarged mitochondria with accumulated Dnm1 and rosary-like mitochondria with Dnm1 foci at constriction sites. These mitochondrial constriction sites retain the continuity of both the outer and inner membranes within an extremely confined space, indicating that Dnm1 is unable to complete mitochondrial fission without Atg44. Moreover, accumulated Atg44 proteins are observed at mitochondrial constriction sites. These findings suggest that Atg44 and Dnm1 cooperatively execute mitochondrial fission from inside and outside the mitochondria, respectively.Abbreviation ATG autophagy related; CLEM correlative light and electron microscopy; EM electron microscopy; ER endoplasmic reticulum; ERMES endoplasmic reticulum-mitochondria encounter structure; GA glutaraldehyde; GFP green fluorescent protein; GTP guanosine triphosphate IMM inner mitochondrial membrane; IMS intermembrane space; OMM outer mitochondrial membrane; PB phosphate buffer; PBS phosphate-buffered saline; PFA paraformaldehyde; RFP red fluorescent protein; WT wild type.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Autophagy
Year:
2024
Document type:
Article
Affiliation country:
Japan