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Engineering cascade biocatalysis in whole cells for syringic acid bioproduction.
Liu, Xin; An, Yi; Gao, Haijun.
Affiliation
  • Liu X; School of Life Science, Beijing Institute of Technology, No 5 Zhongguancun South Street, Haidian District, Beijing, 100081, China.
  • An Y; School of Life Science, Beijing Institute of Technology, No 5 Zhongguancun South Street, Haidian District, Beijing, 100081, China.
  • Gao H; School of Life Science, Beijing Institute of Technology, No 5 Zhongguancun South Street, Haidian District, Beijing, 100081, China. hj_gao@bit.edu.cn.
Microb Cell Fact ; 23(1): 162, 2024 Jun 01.
Article in En | MEDLINE | ID: mdl-38824548
ABSTRACT

BACKGROUND:

Syringic acid (SA) is a high-value natural compound with diverse biological activities and wide applications, commonly found in fruits, vegetables, and herbs. SA is primarily produced through chemical synthesis, nonetheless, these chemical methods have many drawbacks, such as considerable equipment requirements, harsh reaction conditions, expensive catalysts, and numerous by-products. Therefore, in this study, a novel biotransformation route for SA production was designed and developed by using engineered whole cells.

RESULTS:

An O-methyltransferase from Desulfuromonas acetoxidans (DesAOMT), which preferentially catalyzes a methyl transfer reaction on the meta-hydroxyl group of catechol analogues, was identified. The whole cells expressing DesAOMT can transform gallic acid (GA) into SA when S-adenosyl methionine (SAM) is used as a methyl donor. We constructed a multi-enzyme cascade reaction in Escherichia coli, containing an endogenous shikimate kinase (AroL) and a chorismate lyase (UbiC), along with a p-hydroxybenzoate hydroxylase mutant (PobA**) from Pseudomonas fluorescens, and DesAOMT; SA was biosynthesized from shikimic acid (SHA) by using whole cells catalysis. The metabolic system of chassis cells also affected the efficiency of SA biosynthesis, blocking the chorismate metabolism pathway improved SA production. When the supply of the cofactor NADPH was optimized, the titer of SA reached 133 µM (26.2 mg/L).

CONCLUSION:

Overall, we designed a multi-enzyme cascade in E. coli for SA biosynthesis by using resting or growing whole cells. This work identified an O-methyltransferase (DesAOMT), which can catalyze the methylation of GA to produce SA. The multi-enzyme cascade containing four enzymes expressed in an engineered E. coli for synthesizing of SA from SHA. The metabolic system of the strain and biotransformation conditions influenced catalytic efficiency. This study provides a new green route for SA biosynthesis.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Escherichia coli / Biocatalysis / Metabolic Engineering / Gallic Acid Language: En Journal: Microb Cell Fact Journal subject: BIOTECNOLOGIA / MICROBIOLOGIA Year: 2024 Document type: Article Affiliation country: China

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Escherichia coli / Biocatalysis / Metabolic Engineering / Gallic Acid Language: En Journal: Microb Cell Fact Journal subject: BIOTECNOLOGIA / MICROBIOLOGIA Year: 2024 Document type: Article Affiliation country: China