An activatable azophenyl fluorescent probe for hypoxic fluorescence imaging in living cells.
Luminescence
; 39(6): e4798, 2024 Jun.
Article
in En
| MEDLINE
| ID: mdl-38825785
ABSTRACT
Cellular hypoxia is a common pathological process in various diseases. Detecting cellular hypoxia is of great scientific significance for early diagnosis of tumors. The hypoxia fluorescence probe analysis method can efficiently and conveniently evaluate the hypoxia status in tumor cells. These probes are covalently linked by hypoxic recognition groups and organic fluorescent molecules. Currently, the fluorescent molecules used in these probes often exhibit the aggregation-caused quenching effect, which is not conducive to fluorescence imaging in water. Herein, an activatable hypoxia fluorescence probe was constructed by covalently linking aggregation-induced emission luminogens to the hypoxic recognition group azobenzene. It does not emit fluorescence in solution and in solid state under light excitation due to the presence of photosensitive azo bonds. It can be cleaved by intracellular azoreductase into fluorescent amino derivatives with aggregation-induced emission characteristic. As the concentration of oxygen in cells decreases, its fluorescence intensity increases, making it suitable for fluorescence imaging to detect hypoxic environment in live cancer cells. This work broadens the molecular design approach for activatable hypoxia fluorescent probes.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cell Hypoxia
/
Optical Imaging
/
Fluorescent Dyes
Limits:
Humans
Language:
En
Journal:
Luminescence
Journal subject:
BIOFISICA
/
BIOQUIMICA
Year:
2024
Document type:
Article
Affiliation country:
China