Optical projection tomography implemented for accessibility and low cost (OPTImAL).
Philos Trans A Math Phys Eng Sci
; 382(2274): 20230101, 2024 Jul 09.
Article
in En
| MEDLINE
| ID: mdl-38826047
ABSTRACT
Optical projection tomography (OPT) is a three-dimensional mesoscopic imaging modality that can use absorption or fluorescence contrast, and is widely applied to fixed and live samples in the mm-cm scale. For fluorescence OPT, we present OPT implemented for accessibility and low cost, an open-source research-grade implementation of modular OPT hardware and software that has been designed to be widely accessible by using low-cost components, including light-emitting diode (LED) excitation and cooled complementary metal-oxide-semiconductor (CMOS) cameras. Both the hardware and software are modular and flexible in their implementation, enabling rapid switching between sample size scales and supporting compressive sensing to reconstruct images from undersampled sparse OPT data, e.g. to facilitate rapid imaging with low photobleaching/phototoxicity. We also explore a simple implementation of focal scanning OPT to achieve higher resolution, which entails the use of a fan-beam geometry reconstruction method to account for variation in magnification. This article is part of the Theo Murphy meeting issue 'Open, reproducible hardware for microscopy'.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Philos Trans A Math Phys Eng Sci
Journal subject:
BIOFISICA
/
ENGENHARIA BIOMEDICA
Year:
2024
Document type:
Article
Affiliation country:
United kingdom