Concurrent D-loop cleavage by Mus81 and Yen1 yields half-crossover precursors.
Nucleic Acids Res
; 52(12): 7012-7030, 2024 Jul 08.
Article
in En
| MEDLINE
| ID: mdl-38832625
ABSTRACT
Homologous recombination involves the formation of branched DNA molecules that may interfere with chromosome segregation. To resolve these persistent joint molecules, cells rely on the activation of structure-selective endonucleases (SSEs) during the late stages of the cell cycle. However, the premature activation of SSEs compromises genome integrity, due to untimely processing of replication and/or recombination intermediates. Here, we used a biochemical approach to show that the budding yeast SSEs Mus81 and Yen1 possess the ability to cleave the central recombination intermediate known as the displacement loop or D-loop. Moreover, we demonstrate that, consistently with previous genetic data, the simultaneous action of Mus81 and Yen1, followed by ligation, is sufficient to recreate the formation of a half-crossover precursor in vitro. Our results provide not only mechanistic explanation for the formation of a half-crossover, but also highlight the critical importance for precise regulation of these SSEs to prevent chromosomal rearrangements.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Crossing Over, Genetic
/
Saccharomyces cerevisiae Proteins
/
DNA-Binding Proteins
/
Endonucleases
Language:
En
Journal:
Nucleic Acids Res
Year:
2024
Document type:
Article
Affiliation country:
Spain