Hardware and software solutions for implementing nanospray desorption electrospray ionization (nano-DESI) sources on commercial mass spectrometers.
J Mass Spectrom
; 59(7): e5065, 2024 Jul.
Article
in En
| MEDLINE
| ID: mdl-38866597
ABSTRACT
Nanospray desorption electrospray ionization (nano-DESI) is an ambient ionization mass spectrometry imaging (MSI) approach that enables spatial mapping of biological and environmental samples with high spatial resolution and throughput. Because nano-DESI has not yet been commercialized, researchers develop their own sources and interface them with different commercial mass spectrometers. Previously, several protocols focusing on the fabrication of nano-DESI probes have been reported. In this tutorial, we discuss different hardware requirements for coupling the nano-DESI source to commercial mass spectrometers, such as the safety interlock, inlet extension, and contact closure. In addition, we describe the structure of our custom software for controlling the nano-DESI MSI platform and provide detailed instructions for its usage. With this tutorial, interested researchers should be able to implement nano-DESI experiments in their labs.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
J Mass Spectrom
Journal subject:
BIOQUIMICA
Year:
2024
Document type:
Article
Affiliation country:
United States