Protocol for whole-mount immunofluorescence staining of ECM gel-embedded innervated pancreatic organoids.
STAR Protoc
; 5(2): 103132, 2024 Jun 21.
Article
in En
| MEDLINE
| ID: mdl-38875112
ABSTRACT
The mandatory usage of extracellular matrix (ECM) gels in 3D cultures limits antibody penetration and increases background, while the removal of ECM gel causes disruption of morphology and sample loss. These factors pose challenges to effective immune labeling-based staining. Here, we present a protocol for whole-mount immunofluorescence staining of gel-embedded pancreatic organoids. We describe steps for sample fixation, blocking, and antibody incubation. We detail procedures for washing antibodies and mounting.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pancreas
/
Organoids
/
Fluorescent Antibody Technique
/
Extracellular Matrix
Limits:
Animals
/
Humans
Language:
En
Journal:
STAR Protoc
Year:
2024
Document type:
Article
Affiliation country:
Turkey
Country of publication:
United States