Maize auxin response factor ZmARF1 confers multiple abiotic stresses resistances in transgenic Arabidopsis.
Plant Mol Biol
; 114(4): 75, 2024 Jun 15.
Article
in En
| MEDLINE
| ID: mdl-38878261
ABSTRACT
Prolonged exposure to abiotic stresses causes oxidative stress, which affects plant development and survival. In this research, the overexpression of ZmARF1 improved tolerance to low Pi, drought and salinity stresses. The transgenic plants manifested tolerance to low Pi by their superior root phenotypic traits root length, root tips, root surface area, and root volume, compared to wide-type (WT) plants. Moreover, the transgenic plants exhibited higher root and leaf Pi content and upregulated the high affinity Pi transporters PHT1;2 and phosphorus starvation inducing (PSI) genes PHO2 and PHR1 under low Pi conditions. Transgenic Arabidopsis displayed tolerance to drought and salt stress by maintaining higher chlorophyll content and chlorophyll fluorescence, lower water loss rates, and ion leakage, which contributed to the survival of overexpression lines compared to the WT. Transcriptome profiling identified a peroxidase gene, POX, whose transcript was upregulated by these abiotic stresses. Furthermore, we confirmed that ZmARF1 bound to the auxin response element (AuxRE) in the promoter of POX and enhanced its transcription to mediate tolerance to oxidative stress imposed by low Pi, drought and salt stress in the transgenic seedlings. These results demonstrate that ZmARF1 has significant potential for improving the tolerance of crops to multiple abiotic stresses.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plant Proteins
/
Stress, Physiological
/
Plants, Genetically Modified
/
Arabidopsis
/
Gene Expression Regulation, Plant
/
Zea mays
/
Droughts
Language:
En
Journal:
Plant Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
/
BOTANICA
Year:
2024
Document type:
Article
Affiliation country:
China
Country of publication:
Netherlands