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Investigation of variability in the matrix effect on stable isotope-labeled internal standards in liquid chromatography-tandem mass spectrometry analysis of 25 pesticides in vegetables.
Banno, Arisa; Yabuki, Yoshinori; Sonoda, Motohiro; Tanimori, Shinji.
Affiliation
  • Banno A; Department of Environmental Research, Research Institute of Environment, Agriculture, and Fisheries, Osaka Prefecture.
  • Yabuki Y; Department of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University.
  • Sonoda M; Department of Environmental Research, Research Institute of Environment, Agriculture, and Fisheries, Osaka Prefecture.
  • Tanimori S; Department of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University.
J Pestic Sci ; 49(2): 65-76, 2024 May 20.
Article in En | MEDLINE | ID: mdl-38882703
ABSTRACT
The matrix effects (ME) in simultaneous analysis of pesticide residue using liquid chromatography-tandem mass spectrometry (LC-MS/MS) were evaluated by comparing the slopes of matrix-matched and reagent-only calibrations of four types of vegetable samples. Both the sampling and measurement variances of the ME were also determined using one-way analysis of variance. Substantial ion suppression (ME<-20%) was observed in komatsuna, spinach, and tomato when a modified Japanese official method was implemented. The ME magnitude varied significantly due to sample variability for some pesticides, but it varied by no more than 4% as a result of analytical procedure variance. This study also showed that the addition of stable isotope-labeled internal standards at low concentrations improved the recovery of pesticides from samples at various residue levels. The findings of this study highlight the importance and practical application of internal standards and the matrix-matched calibration method in residue analysis using LC-MS/MS.
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: J Pestic Sci Year: 2024 Document type: Article Country of publication: Japan

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: J Pestic Sci Year: 2024 Document type: Article Country of publication: Japan