Specificity of hemorrhagic proteinase from Crotalus atrox (western diamondback rattlesnake) venom.
Biochim Biophys Acta
; 829(1): 127-30, 1985 May 20.
Article
in En
| MEDLINE
| ID: mdl-3888273
ABSTRACT
Hemorrhagic proteinase, HTb, isolated from Crotalus atrox (western diamondback rattlesnake) venom was studied for its specificity. HTb showed fibrinogenase activity, hydrolyzing the A alpha chain of fibrinogen first, followed by the cleavage of the B beta chain. HTb is different from thrombin and did not produce a fibrin clot. The degradation products of fibrinogen were found to be different, indicating that the cleavage sites in the A alpha and B beta chains are different from those of thrombin. N-Benzoyl-Phe-Val-Arg-p-nitroanilide was not hydrolyzed by HTb, although this substrate was hydrolyzed by thrombin and reptilase.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Endopeptidases
/
Metalloendopeptidases
/
Crotalid Venoms
Limits:
Animals
Language:
En
Journal:
Biochim Biophys Acta
Year:
1985
Document type:
Article