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Acrolein Induces Changes in Cell Membrane and Cytosol Proteins of Erythrocytes.
Kopera, Michal; Gwozdzinski, Krzysztof; Pieniazek, Anna.
Affiliation
  • Kopera M; Doctoral School of Exact and Natural Sciences, University of Lodz, 90-236 Lodz, Poland.
  • Gwozdzinski K; Department of Oncobiology and Epigenetics, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, Poland.
  • Pieniazek A; Department of Oncobiology and Epigenetics, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, Poland.
Molecules ; 29(11)2024 May 27.
Article in En | MEDLINE | ID: mdl-38893395
ABSTRACT
High concentrations of acrolein (2-propenal) are found in polluted air and cigarette smoke, and may also be generated endogenously. Acrolein is also associated with the induction and progression of many diseases. The high reactivity of acrolein towards the thiol and amino groups of amino acids may cause damage to cell proteins. Acrolein may be responsible for the induction of oxidative stress in cells. We hypothesized that acrolein may contribute to the protein damage in erythrocytes, leading to the disruption of the structure of cell membranes. The lipid membrane fluidity, membrane cytoskeleton, and osmotic fragility were measured for erythrocytes incubated with acrolein for 24 h. The levels of thiol, amino, and carbonyl groups were determined in cell membrane and cytosol proteins. The level of non-enzymatic antioxidant potential (NEAC) and TBARS was also measured. The obtained research results showed that the exposure of erythrocytes to acrolein causes changes in the cell membrane and cytosol proteins. Acrolein stiffens the cell membrane of erythrocytes and increases their osmotic sensitivity. Moreover, it has been shown that erythrocytes treated with acrolein significantly reduce the non-enzymatic antioxidant potential of the cytosol compared to the control.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Acrolein / Cytosol / Erythrocyte Membrane / Erythrocytes Limits: Humans Language: En Journal: Molecules Journal subject: BIOLOGIA Year: 2024 Document type: Article Affiliation country: Poland Country of publication: Switzerland

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Acrolein / Cytosol / Erythrocyte Membrane / Erythrocytes Limits: Humans Language: En Journal: Molecules Journal subject: BIOLOGIA Year: 2024 Document type: Article Affiliation country: Poland Country of publication: Switzerland