Transient receptor potential mucolipin 1 circumvents oxidative stress in primary human melanocytes.
Skin Res Technol
; 30(6): e13772, 2024 Jun.
Article
in En
| MEDLINE
| ID: mdl-38899729
ABSTRACT
BACKGROUND:
Transient Receptor Potential Mucolipin 1 (TRPML1) serves as a pivotal reactive oxygen species (ROS) sensor in cells, which is implicated in the regulation of autophagy. However, its function in melanocyte autophagy under oxidative stress remains elusive.METHODS:
The expression and ion channel function of TRPML1 were investigated using immunofluorescence and calcium imaging in primary human melanocytes (MCs). After activating TRPML1 with MLSA1 (TRPML1 agonist), autophagy-related molecules were investigated via western blot. ROS level, apoptosis- and autophagy-related molecules were investigated after pretreatment with MLSA1. After interference with TRPML1 expression, mitochondrial structures were visualized by electron microscopy with hydrogen peroxide (H2O2ï¼treatment.RESULTS:
TRPML1 was expressed and functionally active in primary human MCs, and its activation promotes elevated expression of LC3-II and reduced apoptosis and ROS levels under oxidative stress. TRPML1 downregulation caused mitochondrial swelling and disruption of cristae structures under oxidative stress in primary human MCs.CONCLUSIONS:
TRPML1 might mediate lysosomal autophagy in primary human MCs under oxidative stress, participating in mechanisms that maintain the oxidative and antioxidant systems in balance.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Reactive Oxygen Species
/
Oxidative Stress
/
Transient Receptor Potential Channels
/
Melanocytes
Limits:
Humans
Language:
En
Journal:
Skin Res Technol
Journal subject:
DERMATOLOGIA
Year:
2024
Document type:
Article