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Promotion of apoptosis in melanoma cells by taxifolin through the PI3K/AKT signaling pathway: Screening of natural products using WGCNA and CMAP platforms.
Wang, Ye; Chen, Shao-Jie; Ma, Ting; Long, Qiu; Chen, Lan; Xu, Ke-Xin; Cao, Yu.
Affiliation
  • Wang Y; School of Clinical Medicine, Guizhou Medical University, No.9 Beijing Road, Yunyan District, Guiyang 550004, Guizhou, China.
  • Chen SJ; School of Clinical Medicine, Guizhou Medical University, No.9 Beijing Road, Yunyan District, Guiyang 550004, Guizhou, China; Department of Hepatobiliary Surgery, Affiliated Hospital of Guizhou Medical University, No.28 Gui Medical Street, Yunyan District, Guiyang 550004, Guizhou, China.
  • Ma T; School of Clinical Medicine, Guizhou Medical University, No.9 Beijing Road, Yunyan District, Guiyang 550004, Guizhou, China.
  • Long Q; School of Clinical Medicine, Guizhou Medical University, No.9 Beijing Road, Yunyan District, Guiyang 550004, Guizhou, China.
  • Chen L; School of Clinical Medicine, Guizhou Medical University, No.9 Beijing Road, Yunyan District, Guiyang 550004, Guizhou, China.
  • Xu KX; School of Clinical Medicine, Guizhou Medical University, No.9 Beijing Road, Yunyan District, Guiyang 550004, Guizhou, China.
  • Cao Y; School of Clinical Medicine, Guizhou Medical University, No.9 Beijing Road, Yunyan District, Guiyang 550004, Guizhou, China; Department of Dermatology, Affiliated Hospital of Guizhou Medical University, No.28 Gui Medical Street, Yunyan District, Guiyang 550004, Guizhou, China. Electronic address: ca
Int Immunopharmacol ; 138: 112517, 2024 Sep 10.
Article in En | MEDLINE | ID: mdl-38924866
ABSTRACT
Melanoma is a skin cancer originating from melanocytes. The global incidence rate of melanoma is rapidly increasing, posing significant public health challenges. Identifying effective therapeutic agents is crucial in addressing this growing problem. Natural products have demonstrated promising anti-tumor activity. In this study, a plant flavonoid, taxifolin, was screened using Weighted Correlation Network Analysis (WGCNA) in combination with the Connectivity Map (CMAP) platform. Taxifolin was confirmed to inhibit the proliferation, migration, and invasion ability of melanoma A375 and MV-3 cells by promoting apoptosis. Additionally, it suppressed the Epithelial-Mesenchymal Transition (EMT) process of melanoma cells. Cyber pharmacological analysis revealed that taxifolin exerts its inhibitory effect on melanoma through the PI3K/AKT signaling pathway, specifically by downregulating the protein expression of p-PI3K and p-AKT. Notably, the addition of SC-79, an activator of the PI3K/AKT signaling pathway, reversed the effects of taxifolin on cell migration and apoptosis. Furthermore, in vivo experiments demonstrated that taxifolin treatment slowed tumor growth in mice without significant toxic effects. Based on these findings, taxifolin holds promise as a potential drug for melanoma treatment.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Quercetin / Signal Transduction / Cell Movement / Apoptosis / Phosphatidylinositol 3-Kinases / Proto-Oncogene Proteins c-akt / Melanoma Limits: Animals / Humans Language: En Journal: Int Immunopharmacol Journal subject: ALERGIA E IMUNOLOGIA / FARMACOLOGIA Year: 2024 Document type: Article Affiliation country: China

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Quercetin / Signal Transduction / Cell Movement / Apoptosis / Phosphatidylinositol 3-Kinases / Proto-Oncogene Proteins c-akt / Melanoma Limits: Animals / Humans Language: En Journal: Int Immunopharmacol Journal subject: ALERGIA E IMUNOLOGIA / FARMACOLOGIA Year: 2024 Document type: Article Affiliation country: China