Sequencing methods and functional decoding of mRNA modifications.
Fundam Res
; 3(5): 738-748, 2023 Sep.
Article
in En
| MEDLINE
| ID: mdl-38933299
ABSTRACT
More than 160 types of post-transcriptional RNA modifications have been reported; there is substantial variation in modification type, abundance, site, and function across species, tissues, and RNA type. The recent development of high-throughput detection technology has enabled identification of diverse dynamic and reversible RNA modifications, including N6,2'-O-dimethyladenosine (m6Am), N1-methyladenosine (m1A), 5-methylcytosine (m5C), N6-methyladenosine (m6A), pseudouridine (Ψ), and inosine (I). In this review, we focus on eukaryotic mRNA modifications. We summarize their biogenesis, regulatory mechanisms, and biological functions, as well as high-throughput methods for detection of mRNA modifications. We also discuss challenges that must be addressed in mRNA modification research.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Fundam Res
Year:
2023
Document type:
Article
Affiliation country:
China