Comparative physiological, biochemical and transcriptomic analyses to reveal potential regulatory mechanisms in response to starvation stress in Cipangopaludina chinensis.
Comp Biochem Physiol Part D Genomics Proteomics
; 52: 101279, 2024 Jun 24.
Article
in En
| MEDLINE
| ID: mdl-38941864
ABSTRACT
Cipangopaludina chinensis, as a financially significant species in China, represents a gastropod in nature which frequently encounters starvation stress owing to its limited prey options. However, the underlying response mechanisms to combat starvation have not been investigated in depth. We collected C. chinensis under several times of starvation stress (0, 7, 30, and 60 days) for nutrient, biochemical characteristics and transcriptome analyses. The results showed that prolonged starvation stress (> 30 days) caused obvious fluctuations in the nutrient composition of snails, with dramatic reductions in body weight, survival and digestive enzyme activity (amylase, protease, and lipase), and markedly enhanced the antioxidant enzyme activities of the snails. Comparative transcriptome analyses revealed 3538 differentially expressed genes (DEGs), which were significantly associated with specific starvation stress-responsive pathways, including oxidative phosphorylation and alanine, aspartate, and glutamate metabolism. Then, we identified 40 candidate genes (e.g., HACD2, Cp1, CYP1A2, and GPX1) response to starvation stress through STEM and WGCNA analyses. RT-qPCR verified the accuracy and reliability of the high-throughput sequencing results. This study provides insights into snail overwintering survival and the potential regulatory mechanisms of snail adaptation to starvation stress.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Comp Biochem Physiol Part D Genomics Proteomics
/
Compara biochem physiol. D genomics proteomics
/
Comparative biochemistry and physiology. Part D, Genomics & proteomics
Journal subject:
BIOLOGIA
/
GENETICA
Year:
2024
Document type:
Article
Affiliation country:
China
Country of publication:
Netherlands