Immune response regulation by transduced mesenchymal stem cells with decorin gene on bleomycin-induced lung injury, fibrosis, and inflammation.
Allergol Immunopathol (Madr)
; 52(4): 53-59, 2024.
Article
in En
| MEDLINE
| ID: mdl-38970265
ABSTRACT
BACKGROUND:
Pulmonary fibrosis is a pathological hallmark of lung injury. It is an aggressive disease that replaces normal lung parenchyma by fibrotic tissue. The transforming growth factor-beta-mothers against decapentaplegic homolog 3 (TGF-ß1-Smad3) signaling pathway plays a key role in regulating lung fibrosis. Decorin (DCN), a small leucine-rich proteoglycan, has a modulatory effect on the immune system by reversibly binding with TGF-ß and reducing its bioavailability. Mesenchymal stem cell (MSC) therapy is a new strategy that has an immune-modulatory capacity.OBJECTIVE:
The aim of this study was to introduce a new therapeutic approach to harness remodeling in injured lung. MATERIAL ANDMETHODS:
Bone marrow MSCs were isolated and transduced by decorin gene. Lung injury was induced by bleomycin and mice were treated with MSCs, MSCs-decorin, and decorin. Then, oxidative stress biomarkers, remodeling biomarkers, bronchoalveolar lavage cells, and histopathology study were conducted.RESULTS:
Reduced catalase and superoxide dismutase increased due to treatments. Elevated malondialdehyde, hydroxyproline, TGF-ß levels, and polymorphonuclear cells count decreased in the treated groups. Additionally, the histopathology of lung tissues showed controlled inflammation and fibrosis.CONCLUSION:
Transfected decorin gene to MSCs and used cell therapy could control remodeling and bleomycin-induced lung injury.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pulmonary Fibrosis
/
Bleomycin
/
Mesenchymal Stem Cell Transplantation
/
Decorin
/
Mesenchymal Stem Cells
Limits:
Animals
/
Humans
/
Male
Language:
En
Journal:
Allergol Immunopathol (Madr)
Year:
2024
Document type:
Article
Affiliation country:
China