Protocol for performing metabolic pathway-based subtyping of breast tumors.
STAR Protoc
; 5(3): 103173, 2024 Sep 20.
Article
in En
| MEDLINE
| ID: mdl-38970792
ABSTRACT
Here, we present a protocol for analyzing the global metabolic landscape in breast tumors for the purpose of metabolism-based patient stratification. We describe steps for analyzing 1,454 metabolic genes representing 90 metabolic pathways and subjecting them to an algorithm that calculates the deregulation score of 90 pathways in each tumor sample, thus converting gene-level information into pathway-level information. We then detail procedures for performing clustering analysis to identify metabolic subtypes and using machine learning to develop a signature representing each subtype. For complete details on the use and execution of this protocol, please refer to Iqbal et al.1.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Breast Neoplasms
/
Metabolic Networks and Pathways
Limits:
Female
/
Humans
Language:
En
Journal:
STAR Protoc
Year:
2024
Document type:
Article
Country of publication:
United States