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Simultaneous detection of multiple microRNAs based on fluorescence resonance energy transfer under a single excitation wavelength.
Liu, Lin; He, Jia Hui; Wu, Xiao Qiao; Liu, Jia Jun; Lv, Wen Yi; Huang, Cheng Zhi; Liu, Hui; Li, Chun Mei.
Affiliation
  • Liu L; Key Laboratory of Biomedical Analytics (Southwest University), Chongqing Science and Technology Bureau, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, PR China.
  • He JH; Key Laboratory of Biomedical Analytics (Southwest University), Chongqing Science and Technology Bureau, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, PR China.
  • Wu XQ; Key Laboratory of Biomedical Analytics (Southwest University), Chongqing Science and Technology Bureau, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, PR China.
  • Liu JJ; Key Laboratory of Biomedical Analytics (Southwest University), Chongqing Science and Technology Bureau, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, PR China.
  • Lv WY; Key Laboratory of Biomedical Analytics (Southwest University), Chongqing Science and Technology Bureau, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, PR China.
  • Huang CZ; Key Laboratory of Biomedical Analytics (Southwest University), Chongqing Science and Technology Bureau, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, PR China.
  • Liu H; Key Laboratory of Biomedical Analytics (Southwest University), Chongqing Science and Technology Bureau, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, PR China. Electronic address: liuhui78@swu.edu.cn.
  • Li CM; Key Laboratory of Biomedical Analytics (Southwest University), Chongqing Science and Technology Bureau, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, PR China; NMPA Key Laboratory for Quality Monitoring of Narcotic Drugs and Psychotropic Substance, Chongqing 401121, PR
Spectrochim Acta A Mol Biomol Spectrosc ; 322: 124788, 2024 Dec 05.
Article in En | MEDLINE | ID: mdl-38986256
ABSTRACT
MicroRNAs (miRNAs) play a key role in physiological processes, and their dysregulation is closely related to various human diseases. Simultaneous detection of multiple miRNAs is pivotal to cancer diagnosis at an early stage. However, most multicomponent analyses generally involve multiple excitation wavelengths, which are complicated and often challenging to simultaneously acquire multiple detection signals. In this study, a convenient and sensitive sensor was developed to simultaneously detection of multiple miRNAs under a single excitation wavelength through the fluorescence resonance energy transfer between the carbon dots (CDs)/quantum dots (QDs) and graphene oxide (GO). A hybridization chain reaction (HCR) was triggered by miRNA-141 and miRNA-21, resulting in the high sensitivity with a limit of detection (LOD) of 50 pM (3σ/k) for miRNA-141 and 60 pM (3σ/k) for miRNA-21. This simultaneous assay also showed excellent specificity discrimination against the mismatch. Furthermore, our proposed method successfully detected miRNA-21 and miRNA-141 in human serum samples at a same time, indicating its diagnostic potential in a clinical setting.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: MicroRNAs / Fluorescence Resonance Energy Transfer / Quantum Dots / Limit of Detection / Graphite Limits: Humans Language: En Journal: Spectrochim Acta A Mol Biomol Spectrosc Journal subject: BIOLOGIA MOLECULAR Year: 2024 Document type: Article Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: MicroRNAs / Fluorescence Resonance Energy Transfer / Quantum Dots / Limit of Detection / Graphite Limits: Humans Language: En Journal: Spectrochim Acta A Mol Biomol Spectrosc Journal subject: BIOLOGIA MOLECULAR Year: 2024 Document type: Article Country of publication: United kingdom