Runx1 promotes neuronal injury in ischemic stroke through mediating miR-203-3p/Pde4d axis.
Brain Inj
; : 1-11, 2024 Jul 12.
Article
in En
| MEDLINE
| ID: mdl-38994671
ABSTRACT
BACKGROUND:
It has been reported that Runx1 engaged in IS progression, but the detailed mechanism of Runx1 in IS is still unclear.METHODS:
Mice and HT22 cells were subjected to the process of middle cerebral artery occlusion and reperfusion (MCAO/R) and oxygen-glucose deprivation/reoxygenation (OGD/R), respectively. Infract volume was tested using TTC staining. The levels of inflammatory cytokines were investigated using ELISA assay. Cell viability was examined utilizing MTS. Apoptosis rate was evaluated using flow cytometry and TUNEL. The productions of SOD and MDA were monitored by means of commercial kits. The correlations among Runx1, miR-203-3p and Pde4d were ascertained using dual luciferase reporter gene, ChIP and RNA-RNA pull-down assays.RESULTS:
Runx1 and Pde4d were abnormally elevated, while miR-203-3p was notably declined in MCAO/R mice and OGD/R-induced HT22 cells. OGD/R treatment suppressed cell viability and facilitated cell apoptosis, inflammation and oxidative stress, which were compromised by Runx1 knockdown or miR-203-3p upregulation. Runx1 bound to miR-203-3p promoter, thus decreasing miR-203-3p expression. MiR-203-3p inhibited Pde4d expression via targeting Pde4d mRNA. Runx1 deficiency-induced protection effects on OGD/R-treated HT22 cells were offset by miR-203-3p downregulation.CONCLUSION:
Runx1 aggravated neuronal injury caused by IS through mediating miR-203-3p/Pde4d axis.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Brain Inj
Journal subject:
CEREBRO
Year:
2024
Document type:
Article
Publication country:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
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UK
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UNITED KINGDOM