Riboflavin overproduction on lignocellulose hydrolysate by the engineered yeast Candida famata.
FEMS Yeast Res
; 242024 Jan 09.
Article
in En
| MEDLINE
| ID: mdl-39009031
ABSTRACT
Lignocellulose (dry plant biomass) is an abundant cheap inedible residue of agriculture and wood industry with great potential as a feedstock for biotechnological processes. Lignocellulosic substrates can serve as valuable resources in fermentation processes, allowing the production of a wide array of chemicals, fuels, and food additives. The main obstacle for cost-effective conversion of lignocellulosic hydrolysates to target products is poor metabolism of the major pentoses, xylose and L-arabinose, which are the second and third most abundant sugars of lignocellulose after glucose. We study the oversynthesis of riboflavin in the flavinogenic yeast Candida famata and found that all major lignocellulosic sugars, including xylose and L-arabinose, support robust growth and riboflavin synthesis in the available strains of C. famata. To further increase riboflavin production from xylose and lignocellulose hydrolysate, genes XYL1 and XYL2 coding for xylose reductase and xylitol dehydrogenase were overexpressed. The resulting strains exhibited increased riboflavin production in both shake flasks and bioreactors using diluted hydrolysate, reaching 1.5 g L-1.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Riboflavin
/
Xylose
/
Candida
/
Metabolic Engineering
/
Lignin
Language:
En
Journal:
FEMS Yeast Res
Journal subject:
MICROBIOLOGIA
Year:
2024
Document type:
Article
Affiliation country:
Ukraine
Country of publication:
United kingdom