Designing a Multiple-Epitope Vaccine Candidate against Leishmania major and Leishmania infantum for Monocyte-Derived Exosome Preparation.
Iran J Parasitol
; 19(2): 153-161, 2024.
Article
in En
| MEDLINE
| ID: mdl-39011533
ABSTRACT
Background:
Leishmania is a vector-borne protozoon, which causes visceral, cutaneous and mucocutaneous leishmaniosis in human and animals. Monocyte-derived exosome vaccines can be used as prophylaxis and immunotherapy strategies. The aim of this study was to design a multiple-epitope candidate vaccine using leishmaniolysin (GP63) and rK39 proteins against Leishmania major and L. infantum for monocyte-derived exosome preparation.Methods:
This study was carried out in Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran, 2023-2024. Effective immunodominant epitopes were selected from two antigenic proteins of GP63 and rK39 using various immunoinformatics and bioinformatics approaches. Vibrio cholerae ß-subunit was used as an adjuvant to stimulate immune responses. Then, appropriate linkers were selected for the fusion of epitopes. The 3D model of candidate vaccine was predicted and validated.Results:
This designed candidate vaccine could effectively be used as a prophylaxis strategy against leishmaniosis.Conclusion:
A candidate vaccine was designed using bioinformatic and immunoinformatic studies with virtual acceptable quality; however, effectiveness of this vaccine should be verified through further in-vitro and in-vivo studies.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Iran J Parasitol
Year:
2024
Document type:
Article
Affiliation country:
Iran
Country of publication:
Iran