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Simultaneous estimation of rutin and donepezil through RP-HPLC: implication in pharmaceutical and biological samples.
Rana, Rafquat; Mishra, Keerti; Tripathi, Shourya; Gupta, Animesh Kumar; Tiwari, Amrendra Kumar; Yadav, Pavan Kumar; Kumar, Abhiram; Chakradhar, Jvus; Singh, Sanjay; Verma, Sonia; Yadav, Pooja; Chourasia, Manish K.
Affiliation
  • Rana R; Division of Pharmaceutics & Pharmacokinetics, CSIR-Central Drug Research Institute, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow 226031, U.P, India.
  • Mishra K; Jawaharlal Nehru University (JNU), New Delhi 110067, India.
  • Tripathi S; Division of Pharmaceutics & Pharmacokinetics, CSIR-Central Drug Research Institute, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow 226031, U.P, India.
  • Gupta AK; Jawaharlal Nehru University (JNU), New Delhi 110067, India.
  • Tiwari AK; Division of Pharmaceutics & Pharmacokinetics, CSIR-Central Drug Research Institute, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow 226031, U.P, India.
  • Yadav PK; Jawaharlal Nehru University (JNU), New Delhi 110067, India.
  • Kumar A; Division of Pharmaceutics & Pharmacokinetics, CSIR-Central Drug Research Institute, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow 226031, U.P, India.
  • Chakradhar J; Division of Pharmaceutics & Pharmacokinetics, CSIR-Central Drug Research Institute, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow 226031, U.P, India.
  • Singh S; Academy of Scientific & Innovative Research (AcSIR), Ghaziabad 201002, India.
  • Verma S; Division of Pharmaceutics & Pharmacokinetics, CSIR-Central Drug Research Institute, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow 226031, U.P, India.
  • Yadav P; Academy of Scientific & Innovative Research (AcSIR), Ghaziabad 201002, India.
  • Chourasia MK; Division of Pharmaceutics & Pharmacokinetics, CSIR-Central Drug Research Institute, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow 226031, U.P, India.
Bioanalysis ; 16(11): 557-567, 2024 Jun 02.
Article in En | MEDLINE | ID: mdl-39011589
ABSTRACT

Aim:

A HPLC method was developed and validated for the novel combination of rutin (RN) and donepezil (DNP). Materials &

methods:

RN and DNP were simultaneously eluted through a C18 column (Ø 150 × 4.6 mm) with a 6040 v/v ratio of 0.1% formic acid aqueous solution to methanol at 0.5 ml/min.

Results:

The purposed method was found linear, selective, reproducible, accurate and precise with percent RSD less than 2. The limit of quantification for RN and DNP was found 3.66 and 3.25 µg/ml, respectively.

Conclusion:

Validated as per the ICH guidelines, the developed method efficiently quantified RN and DNP co-loaded in DQAsomes (121 nm) estimating matrix effect, release profile, entrapment efficiency, loading efficiency and in vivo plasma kinetics.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Rutin / Donepezil Limits: Humans Language: En Journal: Bioanalysis Year: 2024 Document type: Article Affiliation country: India Country of publication: United kingdom
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Rutin / Donepezil Limits: Humans Language: En Journal: Bioanalysis Year: 2024 Document type: Article Affiliation country: India Country of publication: United kingdom