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Single-cell transcriptomic analysis reveals the antiangiogenic role of Mgarp in diabetic retinopathy.
Ren, Ling; Xia, Jiao; Huang, Chang; Bai, Yun; Yao, Jin; Li, Dan; Yan, Biao.
Affiliation
  • Ren L; Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University, Shanghai, People's Republic of China.
  • Xia J; Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
  • Huang C; Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University, Shanghai, People's Republic of China.
  • Bai Y; College of Information Science, Shanghai Ocean University, Shanghai, People's Republic of China.
  • Yao J; The Affiliated Eye Hospital, Nanjing Medical University, Nanjing, People's Republic of China yanbiao@sjtu.edu.cn jinyao1972@126.com lenslab@163.com.
  • Li D; Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University, Shanghai, People's Republic of China yanbiao@sjtu.edu.cn jinyao1972@126.com lenslab@163.com.
  • Yan B; Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China yanbiao@sjtu.edu.cn jinyao1972@126.com lenslab@163.com.
BMJ Open Diabetes Res Care ; 12(4)2024 Jul 16.
Article in En | MEDLINE | ID: mdl-39013633
ABSTRACT

INTRODUCTION:

Diabetic retinopathy (DR) is a common vascular complication of diabetes mellitus and a leading cause of vision loss worldwide. Endothelial cell (EC) heterogeneity has been observed in the pathogenesis of DR. Elucidating the underlying mechanisms governing EC heterogeneity may provide novel insights into EC-specific therapies for DR. RESEARCH DESIGN AND

METHODS:

We used the single-cell data from the Gene Expression Omnibus database to explore EC heterogeneity between diabetic retinas and non-diabetic retinas and identify the potential genes involved in DR. CCK-8 assays, EdU assays, transwell assays, and tube formation assays were conducted to determine the role of the identified gene in angiogenic effects.

RESULTS:

Our analysis identified three distinct EC subpopulations in retinas and revealed that Mitochondria-localized glutamic acid-rich protein (Mgarp) gene is potentially involved in the pathogenesis of DR. Silencing of Mgarp significantly suppressed the proliferation, migration, and tube formation capacities in retinal endothelial cells.

CONCLUSIONS:

This study not only offers new insights into transcriptomic heterogeneity and pathological alteration of retinal ECs but also holds the promise to pave the way for antiangiogenic therapy by targeting EC-specific gene.
Subject(s)
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Gene Expression Profiling / Endothelial Cells / Diabetic Retinopathy / Single-Cell Analysis Limits: Animals / Humans Language: En Journal: BMJ Open Diabetes Res Care Year: 2024 Document type: Article Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Gene Expression Profiling / Endothelial Cells / Diabetic Retinopathy / Single-Cell Analysis Limits: Animals / Humans Language: En Journal: BMJ Open Diabetes Res Care Year: 2024 Document type: Article Country of publication: United kingdom