Cannabidiol ameliorates lipopolysaccharide-induced cardiovascular toxicity by its antioxidant and anti-inflammatory activity via regulating IL-6, Hif1α, STAT3, eNOS pathway.
Mol Biol Rep
; 51(1): 825, 2024 Jul 18.
Article
in En
| MEDLINE
| ID: mdl-39023749
ABSTRACT
BACKGROUND:
Systemic inflammation causes several organ damage by activating the intracellular signaling mechanisms. Heart and aorta tissues are the structures mostly affected by this situation. By examining underlying processes, this study sought to determine whether cannabidiol (CBD) may have protective effects against the cardiovascular damage brought on by lipopolysaccharide (LPS). MATERIALS ANDMETHODS:
A total of 32 female rats were randomly allocated to one of four groups control, lipopolysaccharide (LPS) (5 mg/kg, i.p., single dose), LPS + CBD (5 mg/kg, i.p., single dose), and CBD groups. The rats were killed six hours after receiving LPS, and tissues from the heart and aorta were taken. Histopathological and immunohistochemical analyzes were performed. Oxidative stress was evaluated biochemically by spectrophotometric method. Expression levels of genes were studied by RT-qPCR method.RESULTS:
Histopathological analysis of the LPS group showed moderate hyperemia, hemorrhages, edema, inflammation, and myocardial cell damage. There was a slight to moderate increase in Cox-1, G-CSF, and IL-3 immunoexpressions, along with enhanced expressions of IL-6, Hif1α, and STAT3 genes, and decreased expressions of eNOS genes. Additionally, there were increased levels of TOS and decreased TAS levels observed biochemically. CBD treatment effectively reversed and improved all of these observed changes.CONCLUSIONS:
CBD protects the heart and aorta against systemic inflammation through its antioxidant and anti-inflammatory activity via regulating IL-6, Hif1α, STAT3, and eNOS intracellular pathways.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cannabidiol
/
Signal Transduction
/
Lipopolysaccharides
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Oxidative Stress
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STAT3 Transcription Factor
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Anti-Inflammatory Agents
/
Antioxidants
Limits:
Animals
Language:
En
Journal:
Mol Biol Rep
Year:
2024
Document type:
Article
Affiliation country:
Turkey