The modulation of the hexosamine biosynthetic pathway impacts the localization of CD36 in macrophages.
Acta Biochim Pol
; 71: 13004, 2024.
Article
in En
| MEDLINE
| ID: mdl-39041003
ABSTRACT
CD36 is a type 2 cell surface scavenger receptor expressed in various tissues. In macrophages, CD36 recognizes oxidized low-density lipoprotein (ox-LDL), which promotes the formation of foam cells, the first step toward an atherosclerotic arterial lesion. CD36 possesses a variety of posttranslational modifications, among them N-glycosylation and O-GlcNAc modification. Some of the roles of these modifications on CD36 are known, such as N-linked glycosylation, which provides proper folding and trafficking to the plasma membrane in the human embryonic kidney. This study aimed to determine whether variations in the availability of UDP-GlcNAc could impact Rab-5-mediated endocytic trafficking and, therefore, the cellular localization of CD36. These preliminary results suggest that the availability of the substrate UDP-GlcNAc, modulated in response to treatment with Thiamet G (TMG), OSMI-1 (O-GlcNAcylation enzymes modulators) or Azaserine (HBP modulator), influences the localization of CD36 in J774 macrophages, and the endocytic trafficking as evidenced by the regulatory protein Rab-5, between the plasma membrane and the cytoplasm.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
CD36 Antigens
/
Macrophages
Limits:
Animals
/
Humans
Language:
En
Journal:
Acta Biochim Pol
Year:
2024
Document type:
Article
Affiliation country:
Mexico