Ethylene oxide suppresses boar sperm function during capacitation.
Reprod Toxicol
; 129: 108678, 2024 10.
Article
in En
| MEDLINE
| ID: mdl-39068997
ABSTRACT
Ethylene oxide (E.O) is an epoxide compound, and it has been utilized as a sterilizer or production of ether compounds in several industries. Although the toxic effects of E.O on bacteria and mammals have been reported, its effects on male reproductive toxicity during sperm capacitation are not fully understood. Therefore, this study was designed to evaluate the effects of E.O exposure during sperm capacitation. Boar spermatozoa were treated with various E.O concentrations (0, 0.1, 1, 10, and 100⯵Ð). After exposure, sperm motility, motion kinematics, capacitation status, intracellular ATP levels, cell viability, expression levels of protein kinase A (PKA) activation, and tyrosine phosphorylation were evaluated. Results revealed that E.O exposure significantly decreased sperm motility, motion kinematics, and intracellular ATP levels but significantly increased the capacitated spermatozoa. In addition, the PKA activation and tyrosine phosphorylation were abnormally changed. According to our results, E.O may cause toxic effects on sperm function during capacitation, which induces male reproductive toxicity. Consequently, we suggest that male reproductive toxicity should be considered when using E.O.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sperm Capacitation
/
Sperm Motility
/
Spermatozoa
/
Adenosine Triphosphate
/
Cyclic AMP-Dependent Protein Kinases
Limits:
Animals
Language:
En
Journal:
Reprod Toxicol
Journal subject:
EMBRIOLOGIA
/
MEDICINA REPRODUTIVA
/
TOXICOLOGIA
Year:
2024
Document type:
Article
Country of publication:
United States