Rapid Analysis of Colonic Metabolomics in High-Fat Diet Mice by Extraction Electrospray Ionization Mass Spectrometry (EESI-MS).
J Agric Food Chem
; 72(32): 18294-18303, 2024 Aug 14.
Article
in En
| MEDLINE
| ID: mdl-39083356
ABSTRACT
Propolis exhibits significant anti-inflammatory, antidiabetic, and antiobesity properties in both mouse models and clinical applications. However, the underlying metabolic mechanisms remain poorly understood. Traditional metabolomic methods that rely on chromatographic separation require complex preprocessing steps and extended detection periods. In this study, we employed extraction electrospray ionization mass spectrometry combined with multivariate analysis to directly profile metabolites in the colon tissue of mice. Our findings demonstrate the efficacy of ethanol extract of propolis (EEP) in mitigating weight gain, reducing inflammatory cytokines, and improving insulin resistance induced by a high-fat diet. Additionally, EEP enhanced glucose tolerance. Through collision-induced dissociation experiments, we identified 26 metabolites, with 4-hydroxyphenylacetic acid, protocatechuic acid, caffeic acid, ferulic acid, hippuric acid, histidine, and tryptophan emerging as potential biomarkers. Notably, tryptophan exhibited the highest content at 8.25 mg/g. Our research facilitates rapid profiling of colon metabolites, underscoring its significant potential for broader applications in animal metabolomic studies.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Propolis
/
Colon
/
Spectrometry, Mass, Electrospray Ionization
/
Metabolomics
/
Diet, High-Fat
/
Mice, Inbred C57BL
Limits:
Animals
/
Humans
/
Male
Language:
En
Journal:
J Agric Food Chem
Year:
2024
Document type:
Article
Affiliation country:
China
Country of publication:
United States